Academic Papers
(AutoPure25) Yuan Tian, et al. Protocol to purify the histone deacetylase SIRT6 and assess its activity in vitro. STAR Protocols. 2023. https://www.cell.com/star-protocols/fulltext/S2666-1667(23)00164-8
2026-08-17 15:54
(AutoPure25) Yuan Tian, et al. Protocol to purify the histone deacetylase SIRT6 and assess its activity in vitro.
STAR Protocols. 2023. https://www.cell.com/star-protocols/fulltext/S2666-1667(23)00164-8
The histone deacetylase known as sirtuin 6 (SIRT6) deacetylates both histone and non-histone proteins but has low deacetylase activity in vitro. Here, we present a protocol to monitor SIRT6-mediated deacetylation of long-chain acyl-CoA synthase 5 in the presence of palmitic acid. We describe the purification of His-SIRT6 and a Flag-tagged substrate. We then detail a deacetylation assay protocol that can be widely applied to study other SIRT6-mediated deacetylation events and the effect of SIRT6 mutations on its activity.....
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