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( Protein Purifier System, Transporter -NaMATE1) Lijing Chang, et al. Complete biosynthesis of nicotine. Cell. 2026.

Highlights • Information-theory-guided omics identifies glycosylation in nicotine biosynthesis • NAMN hydrolase supplies nicotinic acid via an NAD-cycle-independent route • A vacuolar five-component metabolon channels nicotine biosynthesis and transport • MATE transporter enables efficient nicotine engineering in heterologous plants Summary Nicotine, tobacco’s addictive and potent insecticidal alkaloid, has shaped human history, agriculture, and the plants that produce it. However, the enzymatic steps and reaction mechanisms involved in nicotine biosynthesis remain elusive. Here, we reveal that the final coupling reaction is stabilized by glycosylation via a uridine diphosphate (UDP)-glycosyltransferase, reduced and activated by an A622, condensed through a stereoselective intermolecular Mannich-like reaction, sequentially oxidized by a berberine bridge enzyme-like (BBL), and finally deglycosylated by a β-glucosidase to yield nicotine. A 5-component metabolon assembles at vacuolar membranes to channel both nicotine biosynthesis and its transport. We reconstituted this metabolon both in vitro and heterologously in vivo. Abrogating any of these components depletes nicotine accumulations. A multidrug and toxic compound extrusion (MATE) transporter is essential for efficiently engineering nicotine production in heterologous plant species, which confers pest resistance. This work completes the nicotine biosynthesis pathway and provides critical insights into the intermolecular Mannich-like reaction, a fundamental mechanism for scaffold formation in many plant alkaloids.

2026-08-17

( Protein Purifier System, Transporter -NaMATE1) Lijing Chang, et al. Complete biosynthesis of nicotine. Cell. 2026.

( Protein Purifier System, Transporter -NaMATE1) Lijing Chang, et al. Complete biosynthesis of nicotine. Cell. 2026.

2026-08-17

(AutoPre25D, RECEPTOR-NUT & KINASE-BAK1 Protein purificatio）Yifan Bai, et al. Structural basis for the activation of an LRR receptor by a single-disulfide-bridged peptide signal. Plant Communications. 2026.

2026-08-17

(Protein Purifier System) Hujian Lin, et al. Structure and mechanism of the plastid/parasite ATP/ADP translocator. Nature. 2025.

2026-08-17

(AutoTFF075, Exosome-Like Nanovesicles ) Ailin Wu, et al. Honeysuckle-derived nanovesicles alleviate inflammatory bowel disease-associated neuropsychiatric disorders via the gut–brain axis. Theranostics. 2026.

2026-08-27

(AutoTFF075, Exosome-Like Nanovesicles ) Yibo Zheng, et al. Dark tea-derived exosome-like nanovesicles suppress hepatocellular carcinoma via inhibiting Akt pathway and modulating immune profiles. Phytomedicine. 2026.

2026-08-27

(AutoTFF075, Exosome-Like Nanovesicles ) Li P, et al. Honeysuckle-derived exosome-like nanovesicles ameliorated metabolic-associated fatty liver disease by modulating gut microbiota and its metabolites. Nano Research. 2025.

2026-08-27

(AutoTFF075, Exosome-Like Nanovesicles ) Li P, et al. Honeysuckle-derived exosome-like nanovesicles protect against acute liver failure by modulating gut microbiota. International Journal of Nanomedicine. 2025

2026-08-27

(AutoPure100, Protein A) Linjuan Zhou, et al. Preparation technology comparison and performance evaluation of different protein A affinity chromatographic materials. Chinese Journal of Chromatography. 2024.

2026-08-17

(FPLC) Muhammad Naeem, et al. Characterization and rational engineering of Butyrivibrio crossotus argonaute for improved cleavage activity. Biochemical Engineering Journal. 2024.

2026-08-17

(AutoPure100, Enzyme) Donglin Xiang, et al. Purification, characterization of a plant esterase from red kidney bean and its feasibility for pesticide residue analysis in foods. Food Bioscience. 2024.

2026-08-17

(AutoPure100, LtCas12a) Bo Zhou, et al. Purification and functional validation of LtCas12a protein. STAR Protocols. 2023.

2026-08-17

(AutoPure25, Fusion Protein) Yu-Lei Chen, et al. Selection, identification and crystal structure of shark-derived single-domain antibodies against a green fluorescent protein. International Journal of Biological Macromolecules. 2023.

2026-08-17

(AutoPure25) Yuan Tian, et al. Protocol to purify the histone deacetylase SIRT6 and assess its activity in vitro. STAR Protocols. 2023. https://www.cell.com/star-protocols/fulltext/S2666-1667(23)00164-8

2026-08-17

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