Academic Papers
(AutoPure100, Protein A) Linjuan Zhou, et al. Preparation technology comparison and performance evaluation of different protein A affinity chromatographic materials. Chinese Journal of Chromatography. 2024.
2026-08-17 15:54
(AutoPure100, Protein A) Linjuan Zhou, et al. Preparation technology comparison and performance evaluation of different protein A affinity chromatographic materials.
Chinese Journal of Chromatography. 2024. doi: 10.3724/SP.J.1123.2024.01018.
Protein A affinity chromatographic materials are widely used in clinical medicine and biomedicine because of their specific interactions with immunoglobulin G (IgG). Both the characteristics of the matrix, such as its structure and morphology, and the surface modification method contribute to the affinity properties of the packing materials. The specific, orderly, and oriented immobilization of protein A can reduce its steric hindrance with the matrix and preserve its bioactive sites. In this study, four types of affinity chromatographic materials were obtained using agarose and polyglycidyl methacrylate (PGMA) spheres as substrates, and multifunctional epoxy and maleimide groups were used to fix protein A. The effects of the ethylenediamine concentration, reaction pH, buffer concentration, and other conditions on the coupling efficiency of protein A and adsorption performance of IgG were evaluated.
紫外可见分光光度计（UＶ-1900）购于日本岛津公司；蛋白纯化仪（Unique AutoPure 100-L2）购于苏州英赛斯智能科技有限公司。
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