Technical Articles
(AutoTFF075, Exosome-Like Nanovesicles ) Li P, et al. Honeysuckle-derived exosome-like nanovesicles protect against acute liver failure by modulating gut microbiota. International Journal of Nanomedicine. 2025
2026-08-27 10:10
(AutoTFF075, Exosome-Like Nanovesicles ) Li P, et al. Honeysuckle-derived exosome-like nanovesicles protect against acute liver failure by modulating gut microbiota.
International Journal of Nanomedicine. 2025.https://www.dovepress.com/honeysuckle-derived-exosome-like-nanovesicles-protect-against-acute-li-peer-reviewed-fulltext-article-IJN
Purpose: Acute liver failure (ALF) is a rare but life-threatening condition caused by drug toxicity, viral infections, or autoimmune disorders. Current treatments rely heavily on liver transplantation, which is costly and high-risk. As most therapies target single mechanisms, developing safe, cost-effective multi-target drugs is urgently needed.
Methods: In this study, we isolated exosome-like nanovesicles from dried honeysuckle (HNVs) and evaluated their therapeutic potential in a lipopolysaccharide/D-galactosamine (LPS/GalN)-induced ALF mouse model. We first characterized HNVs using cryo-electron microscopy (Cryo-EM), transmission electron microscopy (TEM), and dynamic light scattering (DLS), and confirmed their stability in gastrointestinal simulation fluid in vivo. Subsequently, we validated the biological safety and in vivo distribution of HNVs in mice. Afterwards, we constructed an ALF model and tested the therapeutic efficacy of HNVs on this model. Through RNA sequencing, 16S rRNA analysis, and complementary techniques such as Western blot and quantitative real-time PCR, we elucidated the underlying mechanisms of HNVs in mitigating ALF.
Preparation and Characterization of HNVs
Dried honeysuckle were bought from Tong Ren Tang, a traditional Chinese medicine merchant. Dried honeysuckle was soaked in phosphate buffer solution (PBS) for 30 min, followed by thorough homogenization using a tissue homogenizer. The homogenate was centrifuged at 10,000 rpm for 60 min and the precipitate was discarded. This centrifugation process was repeated several times until the supernatant was free of precipitate visible to the naked eye. The supernatants from multiple centrifugations were combined and used as samples to be processed prior to purification. The above samples were injected into the an Unique AutoTFF075-M Crossflow filtration system (Inscinstech, Suzhou, China) and flowed sequentially through a hollow fiber column with a pore size of 200 nm and a molecular weight cut-off of 750 kDa (CleaSep® Hollow Fiber Filter, HFELA750100640, Membrane Solutions (Nantong) Co. Ltd, China) for hierarchical separation.
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