Technical Articles
(AutoTFF075, Exosome-Like Nanovesicles ) Li P, et al. Honeysuckle-derived exosome-like nanovesicles ameliorated metabolic-associated fatty liver disease by modulating gut microbiota and its metabolites. Nano Research. 2025.
2026-08-27 10:11
(AutoTFF075, Exosome-Like Nanovesicles ) Li P, et al. Honeysuckle-derived exosome-like nanovesicles ameliorated metabolic-associated fatty liver disease by modulating gut microbiota and its metabolites.
Nano Research. 2025. https://www.sciopen.com/article/10.26599/NR.2025.94907986
Metabolic-associated fatty liver disease (MAFLD), a global health burden with limited therapeutic options beyond lifestyle changes, urgently needs novel strategies. We engineered exosome-like nanovesicles (HNVs) from dried honeysuckle (Lonicera japonica), exhibiting significantly more uniform size distribution than conventional herbal extracts and characteristic nanovesicle morphology. Orally delivered HNVs, enriched with bioactive metabolites, dramatically inhibited increased fat vacuoles, lipid droplet deposition, and collagen fibrosis in the livers of mice with MAFLD induced by high-fat diet (HFD). Mechanistically, HNVs orchestrate a dual gut-liver intervention: (1) restoring gut barrier integrity, slashing serum LPS by 1.58-fold and quelling hepatic inflammation; (2) remodeling gut microbiota to suppress bile salt hydrolase (BSH), elevating taurochenodeoxycholic acid (TCDCA) 2.07-fold. This microbial shift reprograms enterohepatic signaling by inhibiting the FXR-FGF15-FGFR4 axis, thereby boosting hepatic cholesterol catabolism via bile acid synthases. Critically, efficacy is strictly microbiota-dependent: abolished by antibiotics and fully transferable via fecal microbiota transplantation (FMT) from HNV-treated donors. Presenting the first natural nanovesicle platform that concurrently targets gut barrier repair and metabolic reprogramming, HNVs establish a pioneering, multi-targeted therapeutic paradigm for MAFLD, directly linking gut microbial ecology to hepatic pathophysiology with high translational potential.
Preparation and characterization of HNVs and honeysuckle decoction (HD)
After purchasing the dried flowers of honeysuckle from Chinese traditional medicine vendor Tong Ren Tang, the concentrated solution was obtained by soaking in 800 mL of PBS and filtering through a strainer after being juiced. The pre-machine sample was obtained by centrifuging the concentrated solution at 10,000 rpm for one hour, then collecting the supernatant. This process was repeated multiple times until no precipitate was visible. The pre-machine sample was poured into a Unique AutoTFF075-M Crossflow filtration system (Inscinstech, Suzhou, China).
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