Technical Articles
(AutoTFF075, Exosome-Like Nanovesicles ) Ailin Wu, et al. Honeysuckle-derived nanovesicles alleviate inflammatory bowel disease-associated neuropsychiatric disorders via the gut–brain axis. Theranostics. 2026.
2026-08-27 10:08
(AutoTFF075, Exosome-Like Nanovesicles ) Ailin Wu, et al. Honeysuckle-derived nanovesicles alleviate inflammatory bowel disease-associated neuropsychiatric disorders via the gut–brain axis.
Theranostics. 2026. doi:10.7150/thno.132241. https://www.thno.org/v16p7419.htm
Rationale: Inflammatory bowel disease (IBD) is frequently accompanied by anxiety and depression, yet effective therapies targeting both intestinal inflammation and neuropsychiatric symptoms remain limited. This study aimed to investigate whether honeysuckle-derived nanovesicles (HNVs) could alleviate IBD-related affective disturbances by modulating the microbial-gut-brain axis (MGBA).
Methods: A chronic colitis mouse model was established using dextran sulfate sodium (DSS). Mice were treated orally with HNVs, and anxiety- and depression-like behaviors were evaluated using behavioral assays. Intestinal barrier integrity, gut microbiota composition, systemic immune responses, proinflammatory cytokine production, hippocampal immune infiltration, 5-hydroxytryptamine (5-HT) biosynthesis and metabolism, and activation of the BDNF/TrkB/GSK3β signaling pathway were assessed using histological, microbiological, immunological, biochemical, and molecular analyses.
Results: Oral administration of HNVs markedly attenuated anxiety- and depression-like behaviors in mice with chronic colitis. HNV treatment preserved intestinal barrier integrity, reshaped the gut microbial community, modulated systemic immune responses, reduced proinflammatory cytokine production and hippocampal inflammatory infiltration, and regulated 5-HT biosynthesis and metabolic pathways. These effects were associated with restoration of intestinal and neural homeostasis and activation of the BDNF/TrkB/GSK3β signaling cascade.
Generation and physicochemical characterization of HNVs:聽Honeysuckle (100 g; purchased from Tongjuntang) was mixed with 1 L of PBS and homogenized using a preset fruit-processing program for 3 min. The resulting mixture was filtered to remove insoluble residues, yielding clarified honeysuckle juice for further processing and characterization. The honeysuckle juice was centrifuged at 4 掳C, 10,000 rpm for 60 min, repeated three times, and the supernatant was collected, and the filter membrane is 0.45 (Millipore MultiScreen庐). Pour the filtered liquid into the crossflow filtration system purification instrument and concentrate it to 50mL through a hollow fiber column of 750 KD (TMP<4 Mpa, AutoTFF075-M; Inscinstech, Suzhou, China). Size and zeta potential characterization relied on the Zetasizer Nano ZSE (Malvern Instruments, United Kingdom). For determining particle concentration, a NanoCoulter counter provided by Resun Technology Co., Ltd. (Shenzhen, China) was utilized.
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